• Shaum Shiyan 1) Programme of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas. Sriwijaya, Indralaya, Sumatera Selatan, Indonesia 2) Faculty of Pharmacy, Universitas Gadjah Mada
  • Triana Hertiani Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, D.I. Yogyakarta, Indonesia.
  • Ronny Martien Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, D.I. Yogyakarta, Indonesia.
  • Akhmad Kharis Nugroho Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, D.I. Yogyakarta, Indonesia.




White tea, Central composite design, Polyphenols, Kinetic-assisted infundation, Epigallocatechin gallate


Objective: This research aimed to find modeling and optimization of a novel kinetic-assisted infundation for rich-epigallocatechin gallate (EGCG) and polyphenols extraction from white tea leaf (Camellia sinensis L.).

Methods: The optimal conditions for the best extraction of kinetic-assisted infundation were determined using central composite design. The content of EGCG, catechin, gallic acid, caffeine, theobromine, and theophylline was quantified using high-performance liquid chromatography with ultraviolet detection (HPLC/UV-detection). The total polyphenolic content (TPC) and total flavonoid content (TFC) was measured using the spectrophotometric method. The semi-purified extract was characterized by HPLC, fourier transform infrared spectrophotometry-universal attenuated total reflectance (FTIR-UATR), and powder-X ray diffraction (P-XRD). The extraction mechanism of target compounds was analyzed using scanning electron microscopy (SEM) qualitatively.

Results: The optimum process for the rich-EGCG and polyphenolic extraction using kinetic-assisted infundation was the concentration of simplicia 14.75 %, the stir rate 440 rpm, and extraction time 12.31 min. The yield of extracts, TPC, TFC, EGCG, caffeine, gallic acid, and theobromine contents in the optimal extraction process were 9.34 %, 70.97 %, 13.95 %, 29.02 %, 11.95 %, 1.33 %, and 0.33 %, respectively.

Conclusion: The kinetic-assisted infundation method proved to be easy to apply with good results as an alternative technique for extracting polyphenolic compounds and rich-EGCG from white tea leaves.


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Tan J, Engelhardt UH, Lin Z, Kaiser N, Maiwald B. Flavonoids, phenolic acids, alkaloids and theanine in different types of authentic Chinese white tea samples. J Food Compos Anal 2017;57:8-15.

Dai W, Xie D, Lu M, Li P, Lv H, Yang C, et al. Characterization of white tea metabolome: Comparison against green and black tea by a nontargeted metabolomics approach. Food Res Int 2017;96:40-5.

Alves MG, Martins AD, Teixeira NF, Rato L, Oliveira PF, Silva BM. White tea consumption improves cardiac glycolytic and oxidative profile of prediabetic rats. J Funct Foods 2015;14:102-10.

Shiyan S, Herlina H, Bella M, Amriani A. Antiobesity and antihypercholesterolemic effects of white tea (Camellia sinensis) infusion on high-fat diet induced obese rats. Pharmaciana 2017;7:278-88.

Almajano MP, Vila I, Gines S. Neuroprotective effects of white tea against oxidative stress-induced toxicity in striatal cells. Neurotox Res 2011;20:372-8.

Hajiaghaalipour F, Kanthimathi MS, Sanusi J, Rajarajeswaran J. White tea (Camellia sinensis) inhibits proliferation of the colon cancer cell line, HT-29, activates caspases and protects DNA of normal cells against oxidative damage. Food Chem 2015;169:401-10.

Shiyan S, Herlina H, Rizkika Sari L. Nephroprotective of anthocyanin pigments extract from red cabbage (Brassica oleracea L. Var. Capitata f. Rubra) against gentamicin-captopril-induced nephrotoxicity in rats. Asian J Pharm Clin Res 2018;11:432.

Zhong J, Xu C, Reece EA, Yang P. The green tea polyphenol EGCG alleviates maternal diabetes–induced neural tube defects by inhibiting DNA hypermethylation. Am J Obstet Gynecol 2016;215:368. e1-368. e10.

Dias TR, Tomas G, Teixeira N, Alves M, Oliveira P, Silva BM. White tea (Camellia sinensis (L.)): antioxidant properties and beneficial health effects. Int J Food Sci Nutr Diet 2013;2:19-26.

Widyaningrum N, Fudholi A, Sudarsono, Setyowati EP. Stability of epigallocatechin gallate (EGCG) from green tea (Camellia sinensis) and its antibacterial activity against staphylococcus epidermidis ATCC 35984 and propionibacterium acnes ATCC 6919. Asian J Biol Sci 2015;8:93-101.

da Silveira TFF, Meinhart AD, Ballus CA, Godoy HT. The effect of the duration of infusion, temperature, and water volume on the rutin content in the preparation of mate tea beverages: an optimization study. Food Res Int 2014;60:241-5.

Lee LS, Lee N, Kim YH, Lee CH, Hong SP, Jeon YW, et al. Optimization of ultrasonic extraction of phenolic antioxidants from green tea using response surface methodology. Molecules 2013;18:13530-45.

Zielinski AAF, Haminiuk CWI, Beta T. Multi-response optimization of phenolic antioxidants from white tea (Camellia sinensis L. Kuntze) and their identification by LC–DAD–Q-TOF–MS/MS. LWT-Food Sci Technol 2016;65:897-907.

Peiro S, Gordon MH, Blanco M, Perez-Llamas F, Segovia F, Almajano MP. Modelling extraction of white tea polyphenols: the influence of temperature and ethanol concentration. Antioxidants 2014;3:684-99.

Li F, Mao YD, Wang YF, Raza A, Qiu LP, Xu XQ. Optimization of ultrasonic-assisted enzymatic extraction conditions for improving total phenolic content, antioxidant and antitumor activities in vitro from Trapa quadrispinosa Roxb. residues. Molecules 2017;22:396-413.

Damiani E, Bacchetti T, Padella L, Tiano L, Carloni P. Antioxidant activity of different white teas: comparison of hot and cold tea infusions. J Food Compos Anal 2014;33:59-66.

Pradeepa M, Kalidas V, Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of Pelargonium graveolens L’her. Int J Appl Pharm 2016;8:7-11.

Pereira SV, Reis RASP, Garbuio DC, Freitas LAP de. Dynamic maceration of Matricaria chamomilla inflorescences: optimal conditions for flavonoids and antioxidant activity. Rev Bras Farmacogn 2018;28:111-7.

Costa Machado ARM, Bastos JK, de Freitas LAP. Dynamic maceration of Copaifera langsdorffi leaves: a technological study using fractional factorial design. Rev Bras Farmacogn 2013;23:79-85.

Zhou J, Zheng X, Yang Q, Liang Z, Li D, Yang X, et al. Optimization of ultrasonic-assisted extraction and radical-scavenging capacity of phenols and flavonoids from Clerodendrum cyrtophyllum Turcz leaves. PLoS One 2013;8:1-8.

Yan L, Xi J. Micro-mechanism analysis of ultrahigh pressure extraction from green tea leaves by numerical simulation. Sep Purif Technol 2017;180:51-7.

Hou W, Zhang W, Chen G, Luo Y. Optimization of extraction conditions for maximal phenolic, flavonoid and antioxidant activity from Melaleuca bracteata leaves using the response surface methodology. PLoS One 2016;11:e0162139.

Cui HY, Murthy HN, Moh SH, Cui YY, Lee EJ, Paek KY. Comparison of conventional and ultrasound-assisted methods for extraction of nutraceutical compounds from Dendrobium candidum. CyTA J Food 2014;12:355-9.

Guo L, Zhu W, Xu F, Liu M, Xie Y, Zhang J. Optimized ultrasonic-assisted extraction of polysaccharides from Cyclina sinensis and evaluation of antioxidant activities in vitro. CyTA J Food 2014;12:32-9.

Alara OR, Abdurahman NH, Olalere OA. Optimization of microwave-assisted extraction of flavonoids and antioxidants from Vernonia amygdalina leaf using response surface methodology. Food Bioprod Proc 2018;107:36-48.

Weissman SA, Anderson NG. Design of experiments (DoE) and process optimization. A review of recent publications. Org Proc Res Dev 2015;19:1605-33.

Bae IK, Ham HM, Jeong MH, Kim DH, Kim HJ. Simultaneous determination of 15 phenolic compounds and caffeine in teas and mate using RP-HPLC/UV detection: method development and optimization of extraction process. Food Chem 2015;172:469-75.

Fernando CD, Soysa P. Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC. Springerplus 2016;5:970.

Grasel F, dos S, Ferrao MF, Wolf CR. Development of methodology for identification the nature of the polyphenolic extracts by FTIR associated with multivariate analysis. Spectrochim Acta Part A 2016;153:94-101.

Cai J, Wang Y, Xi X, Li H, Wei X. Using FTIR spectra and pattern recognition for discrimination of tea varieties. Int J Biol Macromol 2015;78:439-46.

Oliveira RN, Mancini MC, Oliveira FCS de, Passos TM, Quilty B, Thire RM da SM, et al. FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing. Rev Mater 2016;21:767-79.

Nugrahani I, Dillen N. Rapid assay development of diclofenac sodium coated tablet assay using FTIR compared to HPLC method. Int J Appl Pharm 2018;10:43-50.

Louer D. Powder X-ray diffraction, applications. In: Lindon JC, Tranter GE, Koppenaal DW. editors. Encyclopedia of Spectroscopy and Spectrometry (Third Edition), Oxford: Academic Press; 2017. p. 723-31.

Shaik M, Ali Z, Khan M, Kuniyil M, Assal M, Alkhathlan H, et al. Green synthesis and characterization of palladium nanoparticles using Origanum vulgare L. extract and their catalytic activity. Molecules 2017;22:165.

Hernandez C, Ferreira F, Rosa D. X-ray powder diffraction and other analyses of cellulose nanocrystals obtained from corn straw by chemical treatments. Carbohydr Polym 2018;193:39-44.

Taani BA, Khanfar M, Alsoud OA. Enhancement of the release of curcumin by the freeze drying technique using inulin and neusilin as carriers. Int J Appl Pharm 2018;10:42-8.

Ahmad I. Application of ionic liquid as a green solvent for polyphenolics content extraction of Peperomia pellucida (L) kunth herb. J Young Pharm 2017;9:486-90.

Zhang Q, Zhao SH, Chen J, Zhang LW. Application of ionic liquid-based microwave-assisted extraction of flavonoids from Scutellaria baicalensis georgi. J Chromatogr B: Biomed Sci Appl 2015;1002:411-7.

Vinatoru M, Mason TJ, Calinescu I. Ultrasonically assisted extraction (UAE) and microwave assisted extraction (MAE) of functional compounds from plant materials. TrAC, Trends Anal Chem 2017;97:159-78.



How to Cite

Shiyan, S., Hertiani, T., Martien, R., & Nugroho, A. K. (2018). OPTIMIZATION OF A NOVEL KINETIC-ASSISTED INFUNDATION FOR RICH-EGCG AND POLYPHENOLS OF WHITE TEA (CAMELLIA SINENSIS) USING CENTRAL COMPOSITE DESIGN. International Journal of Applied Pharmaceutics, 10(6), 259–267. https://doi.org/10.22159/ijap.2018v10i6.29654



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